2003/3/6  23:26:57   v0.5 wolfgang resch 030306
========================================
CONSTAINTS 
========================================
Max Length:		  50
Min Length:		  25
MinMeltTemp:		78.0C
MinFlankingNucl:	  10
MinGCClamp:		   1
MinGCContent:		40.0


fScore distribution
[-3.00 - -2.20[  *
[-2.20 - -1.40[  *
[-1.40 - -0.60[  **
[-0.60 -  0.20[  *******
[ 0.20 -  1.00[  ******
[ 1.00 -  1.80[  ************
[ 1.80 -  2.60[  ***********************
[ 2.60 -  3.40[  *************************
[ 3.40 -  4.20[  ******************************************************************
[ 4.20 -  5.00[  *

================================================================================
Sequence:  a3l                 
	Pos:  182 wt: G  mut: C
------------------------------------------------------------
GATGACTTTATATCCGCAGGGGCTAGAAATCAACG    wt
...................C...............    mut

Target start position:  163
Target end   position:  197
Length:  35
Distance of first mutation from primer end: 19
Distance of last  mutation from primer end: 15
%GC: 46.0
GC clamp   upstream = 1
GC clamp downstream = 2
Melting temperature: 78.4

 SCORE:  5 / 5
fSCORE:  4.264000
------------------------------------------------------------
ORDERING INFORMATION
5' - GAT GAC TTT ATA TCC GCA GCG GCT AGA AAT CAA CG  -3' , 35 nts
5' - CGT TGA TTT CTA GCC GCT GCG GAT ATA AAG TCA TC  -3' , 35 nts
================================================================================
================================================================================
Sequence:  a3l                 
	Pos:  182 wt: G  mut: C
------------------------------------------------------------
GACTTTATATCCGCAGGGGCTAGAAATCAACGTAC    wt
................C..................    mut

Target start position:  166
Target end   position:  200
Length:  35
Distance of first mutation from primer end: 16
Distance of last  mutation from primer end: 18
%GC: 46.0
GC clamp   upstream = 1
GC clamp downstream = 1
Melting temperature: 78.4

 SCORE:  5 / 5
fSCORE:  4.164000
------------------------------------------------------------
ORDERING INFORMATION
5' - GAC TTT ATA TCC GCA GCG GCT AGA AAT CAA CGT AC  -3' , 35 nts
5' - GTA CGT TGA TTT CTA GCC GCT GCG GAT ATA AAG TC  -3' , 35 nts
================================================================================
================================================================================
Sequence:  a3l                 
	Pos:  182 wt: G  mut: C
------------------------------------------------------------
GACTTTATATCCGCAGGGGCTAGAAATCAACGTACC    wt
................C...................    mut

Target start position:  166
Target end   position:  201
Length:  36
Distance of first mutation from primer end: 16
Distance of last  mutation from primer end: 19
%GC: 47.0
GC clamp   upstream = 1
GC clamp downstream = 2
Melting temperature: 79.8

 SCORE:  5 / 5
fSCORE:  4.123000
------------------------------------------------------------
ORDERING INFORMATION
5' - GAC TTT ATA TCC GCA GCG GCT AGA AAT CAA CGT ACC   -3' , 36 nts
5' - GGT ACG TTG ATT TCT AGC CGC TGC GGA TAT AAA GTC   -3' , 36 nts
================================================================================
================================================================================
Sequence:  a3l                 
	Pos:  182 wt: G  mut: C
------------------------------------------------------------
ACTTTATATCCGCAGGGGCTAGAAATCAACGTAC    wt
...............C..................    mut

Target start position:  167
Target end   position:  200
Length:  34
Distance of first mutation from primer end: 15
Distance of last  mutation from primer end: 18
%GC: 44.0
GC clamp   upstream = 0
GC clamp downstream = 1
Melting temperature: 77.5

 SCORE:  3 / 5
fSCORE:  3.893000
------------------------------------------------------------
ORDERING INFORMATION
5' - ACT TTA TAT CCG CAG CGG CTA GAA ATC AAC GTA C  -3' , 34 nts
5' - GTA CGT TGA TTT CTA GCC GCT GCG GAT ATA AAG T  -3' , 34 nts
================================================================================
================================================================================
Sequence:  a3l                 
	Pos:  182 wt: G  mut: C
------------------------------------------------------------
CTTTATATCCGCAGGGGCTAGAAATCAACG    wt
..............C...............    mut

Target start position:  168
Target end   position:  197
Length:  30
Distance of first mutation from primer end: 14
Distance of last  mutation from primer end: 15
%GC: 47.0
GC clamp   upstream = 1
GC clamp downstream = 2
Melting temperature: 75.8

 SCORE:  4 / 5
fSCORE:  3.296500
------------------------------------------------------------
ORDERING INFORMATION
5' - CTT TAT ATC CGC AGC GGC TAG AAA TCA ACG   -3' , 30 nts
5' - CGT TGA TTT CTA GCC GCT GCG GAT ATA AAG   -3' , 30 nts
================================================================================