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Quickchange primer design
Fasta format sequence(s)
Mutation(s)
Described following these rules:
Each line describes one mutagenesis reaction
Mutations must be close enough together to fit on one primer
Whitespace is ignored
Eeach line contains one sequence name (must be exacly as in fasta file) and one or more of mutations described as : "pos wtNucl mutNucl"
EXAMPLE: foobar 240 G C 245 A T 246 C G